Effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins.

Biologia futura2023PMID: 37067760

View on PubMed DOI: 10.1007/s42977-023-00163-6

Animal StudyMOTS-C

Plain Language Summary

Rat study using intracerebroventricular MOTS-c infusion to examine effects on thyroid axis hormones (TSH, T3, T4) and uncoupling proteins (UCP1, UCP3) in hypothalamus, blood, muscle, and adipose tissue. Investigated whether central MOTS-c signaling modulates thermogenic and thyroid regulatory pathways. Provides the first characterization of central MOTS-c effects on thyroid hormone regulation and uncoupling protein expression—establishing whether hypothalamic MOTS-c signaling coordinates energy balance through thyroid-axis modulation and thermogenesis, relevant for understanding MOTS-c's systemic metabolic regulatory role.

Abstract

This study was conducted to determine the possible effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins (UCPs) in rats. Forty male Wistar Albino rats were divided into 4 groups with 10 animals in each group: control, sham, 10 and 100 µM MOTS-c. Hypothalamus, blood, muscle, adipose tissues samples were collected for thyrotropin-releasing hormone (TRH), UCP1 and UCP3 levels were determined by the RT-PCR and western blot analysis. Serum thyroid hormone levels were determined by the ELISA assays. MOTS-c infusion was found to increase food consumption but it did not cause any changes in the body weight. MOTS-c decreased serum TSH, T3, and T4 hormone levels. On the other hand, it was also found that MOTS-c administration increased UCP1 and UCP3 levels in peripheral tissues. The findings obtained in the study show that central MOTS-c infusion is a directly effective agent in energy metabolism.

Authors

Bahar, Mehmet Refik; Tekin, Suat; Beytur, Asiye; Onalan, Ebru Etem; Ozyalin, Fatma; Colak, Cemil; Sandal, Süleyman

Keywords

Energy useMOTS-cThyroid hormonesUCP1UCP3